| Position | 1st String | 2nd String | 3rd String | 4th String |
|---|---|---|---|---|
| Wr | Jalin Hyatt | Beaux Collins | ||
| Slot | Wan'Dale Robinson | Gunner Olszewski | ||
| RT | Jermaine Eluemunor | Marcus Mbow | ||
| RG | Greg Van Roten | Evan Neal | ||
| C | John Michael Schmitz Jr. | Austin Schlottmann | ||
| LG | Jon Runyan | Aaron Stinnie | ||
| LT | Andrew Thomas | James Hudson III | ||
| RB | Tyrone Tracy Jr. | Devin Singletary | Cam Skattebo | |
| WR | Darius Slayton | Beaux Collins | ||
| TE | Theo Johnson | Daniel Bellinger | Chris Manhertz | Thomas Fidone II |
| QB | Jaxson Dart | Russell Wilson | Jameis Winston | |
| DE | Kayvon Thibodeaux | Chauncey Golston | ||
| NT | Dexter Lawrence II | D.J. Davidson | ||
| DT | Rakeem Nuñez-Roches Sr. | Roy Robertson-Harris | Darius Alexander | |
| OLB | Brian Burns | Abdul Carter | ||
| ILB | Bobby Okereke | Demetrius Flannigan-Fowles | ||
| ILB | Darius Muasau | Swayze Bozeman | ||
| NICKEL | Dru Phillips | Nic Jones | ||
| CB | Paulson Adebo | Art Green | ||
| S | Tyler Nubin | Dane Belton | ||
| S | Jevón Holland | Beau Brade | ||
| CB | Deonte Banks | Cor'Dale Flott | Art Green | |
| LS | Casey Kreiter | |||
| P | Jamie Gillan | |||
| PR | Gunner Olszewski | Wan'Dale Robinson | ||
| KOR | Gunner Olszewski | Wan'Dale Robinson | Devin Singletary | Tyrone Tracy Jr. |
| K | Jude McAtamney | Younghoe Koo |